Pulsed field electrophoresis chambers, accessories and method of utilization for separation of DNA molecules
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Mar 13, 2007
Grant Date -
May 15, 2003
app pub date -
Jun 7, 2001
filing date -
Jun 7, 2000
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Methods of use, accessories and chambers, optimal for performing Pulsed Field Gel Electrophoresis (PFGE) of DNA molecules in `Contour Clamped Homogeneous Electric Field` (CHEF) and `Transversal Alternating Field Electrophoresis` (TAFE) systems, are provided herein. DNA molecules are rapidly separated in the minigels of these chambers. The sizes of chambers and accessories are determined by the separation between the opposite polarity electrodes; which is comprised between 6.2 and 15 cm. Reproducibility of molecule separation is achieved because the accessories warrant homogeneous electric resistance in the buffer and minigels. Chambers allow a high-throughput sample format using the reagents efficiently. It is attained excluding the non-useful electrophoresis zones For a better optimization, TAFE chambers have several useful electrophoresis zones (UEZ), each carrying a minigel. One or more UEZ can be activated at will in the electrophoresis, to vary the number of minigels, the number of samples and the amount of buffer among the experiments. TAFE chambers having `inverted electrode configuration` with the cathodes at their bottom are presented.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Advertisement
Advertisement
Advertisement
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully