Drosophila clipped FRT (cFRT) chromosome insensitive to P transposase, generating method thereof, and application thereof
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United States of America Patent
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Mar 13, 2007
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app pub date -
Oct 20, 2003
filing date -
Jan 10, 2002
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Abstract
A method for generating a Drosophila clipped FRT (cFRT) chromosome is provided, wherein the chromosome is insensitive to a P transposase but remains functional to a yeast site-specific flippase recombinase (FLP). The method includes steps of: (a) exposing a FRT chromosome to the P transposase for occurring a local and imprecise transposition, wherein the FRT chromosome contains a P[FRT] insertion with a selection marker gene, (b) screening the P[FRT] insertion insensitive to the P transposase to obtain screened products, (c) selecting candidate products from the screened products by further examinations, and (d) exposing the candidate products by the P transposase and selecting a desired product by the further examinations to obtain the Drosophila clipped FRT (cFRT) chromosome insensitive to the P transposase but remaining functional to the yeast site-specific flippase recombinase. The cFRT.sup.2L2R chromosome can be used as the direct target in the direct P-transposon-induced mutagenesis.
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