Rapid identification of bacteria from positive blood cultures
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United States of America Patent
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Apr 17, 2007
Grant Date -
Feb 17, 2005
app pub date -
Jul 23, 2004
filing date -
Jul 24, 2003
priority date (Note) -
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Abstract
Disclosed is a method of detecting bacteria in a biological sample, especially a blood sample, without the need for extensive sub-culturing of the sample. Nucleic acid present within the sample is isolated and bacterial DNA specifically amplified using primers that uniquely prime the amplification of 16s rRNA-encoding nucleic acid. The amplicons are then digested with an endonuclease to yield a restriction fragment length profile for the biological sample. The restriction fragment length profile for the biological sample is then compare to a database of profiles made using cultures of known bacterial species. A match between the sample profile and the database identifies the bacteria present in the sample.
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