Method of sequencing a nucleic acid
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United States of America Patent
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May 1, 2007
Grant Date -
Dec 9, 2004
app pub date -
Feb 26, 2004
filing date -
Sep 16, 1999
priority date (Note) -
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Abstract
Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.
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