Molecular torches and their use under strand displacement conditions
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United States of America Patent
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Jul 31, 2007
Grant Date -
Jun 16, 2005
app pub date -
Jan 27, 2005
filing date -
Jul 2, 1998
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Abstract
The present invention features 'molecular torches' and the use of molecular torches for detecting the presence of a target nucleic acid sequence. Molecular torches contain a target binding domain, a target closing domain, and a joining region. The target binding domain is biased towards the target sequence such that the target binding domain forms a more stable hybrid with the target sequence than with the target closing domain under the same hybridization conditions. The joining region facilitates the formation or maintenance of a closed torch.
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