Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes
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United States of America Patent
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Aug 7, 2007
Grant Date -
Mar 18, 2004
app pub date -
Jul 2, 2003
filing date -
Jul 2, 2002
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Abstract
This invention provides polyanionic polymer conjugates containing non-nucleotide polyanionic polymers that are useful in detecting target analytes such as proteins or small molecules. The invention also provides nanoparticles bound to polyanionic polymer conjugates and methods of preparation and use thereof. The polyanionic polymer conjugates have the formula: L-O--[PO.sub.2--O-Z-O].sub.n--PO.sub.2--O--X wherein n ranges from 1 to 200; L represents a moiety comprising a functional group for attaching the polyanion polymer to the nanoparticle surface; Z represents a bridging group, and X represents Q, X' or -Q-X', wherein Q represents a functional group for attaching a recognition probe to the polyanion polymer, and X' represents a recognition probe.
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