Cyanine compounds and their application as quenching compounds
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United States of America Patent
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Sep 18, 2007
Grant Date -
Sep 1, 2005
app pub date -
Aug 11, 2004
filing date -
Aug 11, 2003
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Abstract
The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: ##STR00001## Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or unsubstituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.
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