Methods for using the osteoclastogenesis inhibitory factor (OCIF) protein
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United States of America Patent
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Oct 2, 2007
Grant Date -
Jul 22, 2004
app pub date -
Feb 25, 2004
filing date -
Feb 20, 1995
priority date (Note) -
Expired
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Abstract
A protein which inhibits osteoclast differentiation and/or maturation and a method of production of the protein. The protein is produced by human embryonic lung fibroblasts and has molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis, respectively.The protein can be isolated and purified from culture medium of the said fibroblasts. Furthermore, the protein can be produced by gene engineering.The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity to the protein or a method for determination of the protein concentration using the antibodies.
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