Genetically modified cells and methods for converting (R)-methylmalonyl CoA to (S)-methylmalonyl CoA
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United States of America Patent
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Nov 6, 2007
Grant Date -
Sep 23, 2004
app pub date -
Apr 21, 2004
filing date -
Oct 27, 1999
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Abstract
Recombinant Escherichia coli host cells that comprise recombinant DNA expression vectors that drive expression of methylmalonyl CoA mutase from Propionibacterium shermanii or Streptomyces cinnamonensis as well as Propionibacterium shermanii epimerase can produce S-methylmalonyl CoA, a required substrate for the production of polyketides by most modular polyketide synthases not present in wild-type E. coli host cells.
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