Method of preparing polynucleotide fragments for use in shuffling, and shuffling of same
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United States of America Patent
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Dec 4, 2007
Grant Date -
Oct 28, 2004
app pub date -
Nov 17, 2003
filing date -
May 17, 2001
priority date (Note) -
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Abstract
The invention relates to a fragmentation process that depends on mismatches between two strands of parental polynucleotides. One embodiment, comprises a method for preparing polynucleotide fragments of DNA comprising formation of heteroduplex molecules by hybridizing polynucleotides. The invention also provides a method and process of forming fragments which can be used with any shuffling process or combination of shuffling processes.
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