Process for the in vitro culture of different stages of tissular parasites
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United States of America Patent
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Jan 8, 2008
Grant Date -
Mar 27, 2003
app pub date -
Oct 1, 2002
filing date -
May 13, 1993
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The process of the invention comprises the implementation of axenic conditions, with use of a liquid single-phase culture medium. For obtaining the amastigote forms, this medium is buffered at a pH of 5.5 to 6.5 and has an osmolarity of at least 400 milliosmoles/kg of liquid, and in particular 400 to 550 milliosmoles/kg of liquid. For obtaining promastigote forms, this medium is buffered at a pH of 7 to 7.5 and has an osmolarity of at least 300 milliosmoles/kg of liquid. This process allows the adaptation and culture in vitro of different stages of tissular parasites, such as leishmanias and T. cruzi or also hematoprotozoa.
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