Method for reducing effect of hematocrit on measurement of an analyte in whole blood
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United States of America Patent
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Jan 29, 2008
Grant Date -
Aug 12, 2004
app pub date -
Feb 2, 2004
filing date -
Feb 11, 2003
priority date (Note) -
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Abstract
A method for determining the presence and/or amount of an analyte in a sample of whole blood comprises the step of treating the sample with a nonlytic hypertonic salt composition to reduce the hematocrit by reducing the size of the red blood cells. In optical detection systems, the smaller red blood cells create greater scatter, which allows a more accurate correction to be applied in a dual-wavelength detection system. In electrochemical detection systems, as well as in optical detection systems, the smaller red blood cells provide less obstruction to the diffusion of analyte and reagents in the sample, to facilitate the reactions thereof.
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