Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vectors
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United States of America Patent
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Jan 29, 2008
Grant Date -
Nov 25, 2004
app pub date -
May 29, 2002
filing date -
Jun 8, 2001
priority date (Note) -
Expired
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Abstract
Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.
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