Methods for detecting methylated nucleotides
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United States of America Patent
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Feb 19, 2008
Grant Date -
Sep 25, 2003
app pub date -
Oct 15, 2002
filing date -
Oct 13, 1999
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Abstract
A method for detecting methylated nucleotides within a nucleic acid sample is provided. The method includes the steps of splitting a nucleic acid sample into separate reaction vessels; contacting nucleic acid in one reaction vessel with bisulfite; amplifying the nucleic acid in each reaction vessel; cleaving the nucleic acids in each reaction vessel to produce fragments thereof; and obtaining a mass spectrum of the resulting fragments from one reaction vessel and another mass spectrum of the resulting fragments from another reaction vessel.
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