Methods for detecting genetic mosaicisms using arrays
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United States of America Patent
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Apr 1, 2008
Grant Date -
Jul 3, 2003
app pub date -
Sep 27, 2002
filing date -
Sep 27, 2001
priority date (Note) -
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Abstract
Disclosed are methods for identifying genetic mosaicisms in cell populations. Suitable cell populations include, e.g., biopsy or body fluid samples or cultures of cancer cells. The method includes performing array-based comparative genomic hybridization (CGH), wherein a plurality of cloned genomic nucleic acid segments is provided in a plurality of identical replicas, each cloned segment immobilized to a discrete and known spot on a substrate surface to form the array, and the cloned genomic nucleic acid segments comprise a substantially complete first genome of a known first karyotype. The invention also provides methods for optimizing performance of an array-based comparative genomic hybridization (CGH).
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