Method for treating biomass for producing cell lysate containing plasmid DNA
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United States of America Patent
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May 27, 2008
Grant Date -
Jan 22, 2004
app pub date -
Dec 24, 2001
filing date -
Jan 19, 2001
priority date (Note) -
Expired
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Abstract
The method of making the clear cell lysate containing plasmid DNA from a biomass obtained in a cell culture process includes filtering the biomass obtained in the cell culture process with a filter medium in the presence of diatomaceous earth acting as a filtering agent to form a filter cake containing the biomass and the filter medium, then thermally digesting the biomass in the filter cake to form a cell lysate containing the plasmid DNA and filtering the cell lysate to form a clear filtrate. The clear filtrate contains the plasmid DNA and has a clarity characterized by an OD.sub.600 of at maximum 0.05 U/cm.
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