Method for cultivating cancer cells from human tissue and device for preparing tissue samples
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United States of America Patent
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Jul 1, 2008
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N/A
app pub date -
Feb 18, 2000
filing date -
Mar 10, 1999
priority date (Note) -
Expired
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Abstract
The invention relates to a method for cultivating cancer cells for scientific serial assays, wherein a tissue sample which is heterogeneous with respect to contaminants, normal cells and tumor cells is locally separated in a sequential-parallel splitting method. The locally separated sample segments are further split, wherein the tissue fragments and liquids of the tissue segments are separately placed in a given cell culture medium and grown under predetermined culture conditions. The invention also relates to a cell culture medium and a device for splitting the tissue samples into disc segments. The inventive method combined with the splitting device and the culture medium enables fast cultivation of cancer cells obtained from human tissue with a multiplication rate of 100% in all types of tumors.
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