Multiplexing assays for analyte detection
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United States of America Patent
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Sep 9, 2008
Grant Date -
Jan 5, 2006
app pub date -
Jun 10, 2005
filing date -
Jun 10, 2004
priority date (Note) -
Expired
status (Latency Note)
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Abstract
High density multiplexing assays for proteins, nucleic acids, and other molecules are described based upon using mass spectrometry detection. A solid-state support assay format is utilized, whereby, the bead carries both a coding molecule to track the sample or analyte and the molecule(s) necessary to perform an affinity-based assay. After the necessary reactions are complete, all of the components, e.g., coding molecule, binding molecule, analyte, and/or label are dissociated for analysis in a mass spectrometer. Various means of performing detection for the assays include different types of mass spectrometers with different types of sampling systems, including MALDI-TOF and ESI.
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