Nucleic acid molecules encoding osteoclastogenesis inhibitory factor proteins
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United States of America Patent
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Dec 23, 2008
Grant Date -
Feb 3, 2005
app pub date -
Aug 31, 2004
filing date -
Feb 20, 1995
priority date (Note) -
Expired
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Abstract
A protein which inhibits osteoclast differentiation and/or maturation and a method for producing the protein. The protein is produced by human embryonic lung fibroblasts and has a molecular weight of about 60 kD and about 120 kD under non-reducing conditions and about 60 kD under reducing conditions on SDS-polyacrylamide gel electrophoresis. The protein can be isolated and purified from the culture medium of fibroblasts. Furthermore, the protein can be produced by gene engineering. The present invention includes cDNA for producing the protein by gene engineering, antibodies having specific affinity for the protein or a method for determining protein concentration using these antibodies.
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