Mortierella alpina C.sub.16/18 fatty acid elongase
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United States of America Patent
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Dec 30, 2008
Grant Date -
Apr 19, 2007
app pub date -
Oct 19, 2005
filing date -
Oct 19, 2005
priority date (Note) -
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Abstract
The present invention relates to a fungal C.sub.16/18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C.sub.16/18 fatty acid elongase is provided (designated as 'ELO3'). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C.sub.16/18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.
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