Methods for promoting maturation of conifer somatic embryos
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United States of America Patent
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Apr 21, 2009
Grant Date -
Jan 6, 2005
app pub date -
Jun 4, 2004
filing date -
Jun 23, 2003
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Abstract
In one aspect, the present invention provides methods for producing mature conifer somatic embryos. The methods of the invention comprise the steps of: (a) culturing pre-cotyledonary conifer somatic embryos in, or on, a development medium comprising less than 0.5% of gellan gum to form cotyledonary conifer somatic embryos; and (b) culturing cotyledonary conifer somatic embryos formed in step (a) in, or on, a maturation medium comprising between about 0.5% to about 1.5% of gellan gum to form mature conifer somatic embryos. In some embodiments, the maturation medium further comprises sucrose and/or abscisic acid.
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