Gene carriers with the use of polysaccharide and process for producing the same

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United States of America Patent

PATENT NO 7521433
APP PUB NO 20060084149A1
SERIAL NO

10471366

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Disclosed are gene carriers utilizing β-1,3-glucan and methods of preparing the same. The β-1,3-glucan has at least one 1,6-glucopyranoside branch and is chemically modified through periodate oxidation and reductive amination so as to impart nucleic acid-binding functional groups (for example, cationic functional groups) to at least some of the 1,6-glucopyranoside branches thereof. Triple helix β-1,3-glucan is dissolved in a polar organic solvent to form single-stranded β-1,3-glucan. By replacing, in the presence of a nucleic acid, the polar organic solvent for the solution containing the chemically modified single-stranded β-1,3-glucan by water, a complex (gene carrier) is formed in which the nucleic acid is bound to double-stranded β-1,3-glucan.

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Patent Owner(s)

  • FUKUOKA PREFECTURAL GOVERNMENT;JAPAN SCIENCE AND TECHNOLOGY AGENCY;MITSUI SUGAR CO., LTD.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kimura, Taro Dazaifu , JP 24 147
Koumoto, Kazuya Kurume , JP 5 36
Mizu, Masami Sakai , JP 7 21
Sakurai, Kazuo Himeji , JP 47 429
Shinkai, Seiji Fukuoka , JP 19 202

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