Reagent system and process for adenosine triphosphate monitoring
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United States of America Patent
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Jul 7, 2009
Grant Date -
Apr 6, 2006
app pub date -
Oct 1, 2004
filing date -
Oct 1, 2004
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Abstract
A reagent system comprises a first reagent which includes a high pH phosphate buffer, and a second reagent which includes luciferase, luciferin, a magnesium salt and an enzyme stabilizer. The second reagent has a low pH and a buffer with a pK which is near the optimum pH for activity of luciferase. The reagent system may be used in a process for measuring total adenosine triphosphate (ATP) and/or dissolved extracellular ATP, in a fluid containing microorganisms. The reagent system may also be used in a microbiological remediation or production process.
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