Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
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United States of America Patent
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Aug 4, 2009
Grant Date -
Mar 22, 2007
app pub date -
Sep 1, 2006
filing date -
Sep 7, 2005
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Expired
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Abstract
The present invention deals with a method of preparing nucleic acids, particularly RNA, from a whole blood sample. The nucleic acids purified by the method of the invention are particularly suited for detection of nucleic acid marker molecules. Preferred are markers for the diagnosis of transmissible spongiform encephalopathy (TSE). Such diagnosis is based on the detection, by means of real-time PCR, of certain mRNAs of the TSE-infected organism. Said mRNAs specifically originate as splicing variants and are isolated from whole blood by the method of the invention.
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