DNA joining method
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United States of America Patent
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Aug 18, 2009
Grant Date -
Aug 28, 2003
app pub date -
Mar 7, 2001
filing date -
Mar 7, 2000
priority date (Note) -
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Abstract
The present invention provides a method to directionally clone any linear template DNA molecule into any linearized vector. The vector ends may be generated from any restriction enzyme cleavage. The method does not require a ligation step nor the use of carefully controlled conditions as is required with methods involving specific exonucleases alone. It has been determined that specific DNA polymerases are able to efficiently join one or more linear DNA molecules sharing ends with appropriate complementation.
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