Method for quantitative PCR data analysis system (QDAS)
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United States of America Patent
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Sep 8, 2009
Grant Date -
Jan 12, 2006
app pub date -
Jul 6, 2004
filing date -
Jul 6, 2004
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Abstract
This invention provides a method for the quantitation and quality assurance of QPCR data. In particular, the invention provides a method for extracting data curves from a QPCR assay, estimating the shape and characteristics of an amplification curve and producing a quality score metric. The invention also provides a robust method for calculating a threshold cycle (C.sub.T) value and classifying the status of the QPCR results.
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