Device for extracting biological molecules from tissue specimens and methods for preparing the same
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United States of America Patent
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Sep 15, 2009
Grant Date -
Oct 20, 2005
app pub date -
Apr 15, 2005
filing date -
Apr 16, 2004
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention provides a non-destructive macromolecules extraction (NDME) device for extracting high quantity of biological molecules (including, but not limited to, proteins, DNA, and/or RNA) from tissue specimens (including, but not limited to, fresh or fixed tissue sections, homogenized tissues, and cell cultures), while preserving the morphology and antigenicity of the tissue. The device contains a base, a slide cover, and a thermal control device. The tissue specimen is placed onto the base. The slide cover is mounted to the base to form a space where an extraction solution can be added. The device optionally contains a chamber cover over the slide cover. The chamber cover and the slide cover forms a reaction chamber where a steam is infused to maintain the humidity of the tissue. The extraction solution contains a detergent. The present invention also provides a method for extracting the same.
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