Compositions and methods for inhibiting light-induced inactivation of biological reagents
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United States of America Patent
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Sep 15, 2009
Grant Date -
Jul 10, 2003
app pub date -
Jan 7, 2003
filing date -
Apr 9, 1997
priority date (Note) -
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Abstract
Disclosed is a fluorescent conjugate comprising a biological reagent, a fluorescent molecule, and a means for impeding phototoxic degradation of the biological reagent. The impeding means can be a cross-linking substance having a long molecular distance, whereby the cross-linking substance links the fluorescent molecule and the biological reagent; a quencher of singlet oxygen; a quencher of a free radical; or a combination thereof. Also disclosed is a solution comprising a fluorescent conjugate of a biological reagent and a fluorescent molecule together with an oxygen depleting system.
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