Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
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United States of America Patent
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Oct 6, 2009
Grant Date -
Jan 1, 2004
app pub date -
Mar 21, 2003
filing date -
Apr 9, 2002
priority date (Note) -
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Abstract
The present invention provides methods for producing cotyledonary pine embryos. The methods of the invention each include the step of culturing embryogenic pine tissue in, or on, a medium comprising a disaccharide and glucose to yield cotyledonary pine embryos, wherein the disaccharide and glucose are each present in the medium at a concentration of less than 3 percent. The present invention also provides cotyledonary pine embryos prepared by a method of the invention.
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