Methods and apparatus for screening and detecting multiple genetic mutations
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United States of America Patent
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Oct 13, 2009
Grant Date -
Jul 29, 2004
app pub date -
Jul 24, 2003
filing date -
Jul 26, 2002
priority date (Note) -
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Abstract
An assay system and methods are described where patient samples containing genomic DNA are analyzed for the presence of known genetic polymorphisms using a universal reporter strategy. In a preferred embodiment, the amplified DNA is localized at test sites in an array of sites on a microchip followed by a series of hybridization reactions that screen for the presence of a single mutation from among a number of mutations, and allow the identification of specific mutations. In addition to universal reporters, the assay may use blockers and discriminators for screening and identification of known polymorphisms.
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