Mutan endonuclease with substrate-specific cleavage activity
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United States of America Patent
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Dec 15, 2009
Grant Date -
May 3, 2007
app pub date -
Oct 2, 2006
filing date -
Oct 24, 2005
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Abstract
A novel mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but showing a specific activity. The present invention relates to a mutant endonuclease V not exhibiting a nonspecific nucleic acid cleavage activity but having a specific activity, to a gene coding for the endonuclease V, to a recombinant DNA containing the gene, to a transformant or transductant containing the recombinant DNA, and to a method for producing the endonuclease V. The present invention also relates to a method of using the endonuclease V for nucleic acid cleavage, and to a reagent kit for detection or modification of a nucleic acid or gene that contains the endonuclease V.
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