Amplified signal in binding assays
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United States of America Patent
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Dec 22, 2009
Grant Date -
Sep 9, 2004
app pub date -
Dec 7, 2000
filing date -
Dec 7, 2000
priority date (Note) -
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status (Latency Note)
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Abstract
Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.
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