Three dimensional format biochips
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United States of America Patent
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Dec 29, 2009
Grant Date -
Feb 17, 2005
app pub date -
Oct 25, 2001
filing date -
Apr 26, 1999
priority date (Note) -
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Abstract
A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Binding entities are immobilized in these cells, which entities are effective to selectively hybridize to or sequester a target biomolecule. Different binding entities are immobilized in different cells in an array to create a biochip that can be used to assay for a number of target biomolecules.
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