Methods for concurrent identification and quantification of an unknown bioagent
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United States of America Patent
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Feb 23, 2010
Grant Date -
Jan 1, 2009
app pub date -
Feb 17, 2005
filing date -
Feb 18, 2004
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Abstract
The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon.
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