Saponin-decomposing enzyme, gene thereof and large-scale production sytem for producing soyasapogenol B
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United States of America Patent
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Mar 2, 2010
Grant Date -
Sep 10, 2009
app pub date -
Apr 3, 2009
filing date -
Jun 6, 2001
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
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