Methods for storing conifer somatic embryo germinants
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United States of America Patent
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Mar 30, 2010
Grant Date -
Jun 28, 2007
app pub date -
Dec 1, 2006
filing date -
Dec 22, 2005
priority date (Note) -
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Abstract
In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium.
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