US Patent No: 7,692,063
Number of patents in Portfolio can not be more than 2000
Production of foreign nucleic acids and polypeptides in sprout systems
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Apr 6, 2010
Issued date -
Feb 13, 2006
filing date -
11/353,905
serial no -
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Abstract
The present invention provides systems and methods for producing a nucleic acid or protein in transgenic sprouted seedlings or sprouted seedlings engineered to transiently express a nucleic acid or protein of interest. The sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter or a viral promoter. The sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the nucleic acid or protein.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
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| 5,316,931 Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes | 71 | 1992 | |
| 5,811,653 Viral amplification of recombinant messenger RNA in transgenic plants | 17 | 1993 | |
| 5,589,367 Recombinant plant viral nucleic acids | 34 | 1994 | |
| 5,965,794 Viral amplification of recombinant messenger RNA in transgenic plants | 13 | 1994 | |
| 5,866,785 Recombinant plant viral nucleic acids | 24 | 1995 | |
| 5,889,190 Recombinant plant viral nucleic acids | 14 | 1995 | |
| 5,889,191 Viral amplification of recombinant messenger RNA in transgenic plants | 7 | 1995 | |
| 6,054,566 Recombinant animal viral nucleic acids | 6 | 1995 | |
| 6,660,500 Production of peptides in plants as viral coat protein fusions | 5 | 2001 | |
| 6,740,740 Process for isolating and purifying proteins and peptides from plant sources | 10 | 2001 | |
| 6,841,659 Method for recovering proteins from the interstitial fluid of plant tissues | 9 | 2003 | |
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| 5,855,866 Methods for treating the vasculature of solid tumors | 133 | 1994 | |
| 5,965,132 Methods and compositions for targeting the vasculature of solid tumors | 51 | 1994 | |
| 5,877,289 Tissue factor compositions and ligands for the specific coagulation of vasculature | 91 | 1995 | |
| 6,004,555 Methods for the specific coagulation of vasculature | 57 | 1995 | |
| 6,093,399 Methods and compositions for the specific coagulation of vasculature | 51 | 1995 | |
| 6,261,535 Diagnostic methods for targeting the vasculature of solid tumors | 52 | 1998 | |
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| 5,569,825 Transgenic non-human animals capable of producing heterologous antibodies of various isotypes | 306 | 1991 | |
| 5,633,425 Transgenic non-human animals capable of producing heterologous antibodies | 280 | 1992 | |
| 5,545,806 Ransgenic non-human animals for producing heterologous antibodies | 363 | 1992 | |
| 5,661,016 Transgenic non-human animals capable of producing heterologous antibodies of various isotypes | 263 | 1993 | |
| 5,625,126 Transgenic non-human animals for producing heterologous antibodies | 281 | 1994 | |
| 5,770,429 Transgenic non-human animals capable of producing heterologous antibodies | 142 | 1995 | |
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| 7,012,172 Virus induced gene silencing in plants | 4 | 2002 | |
| 2005/0026,291 System for expression of genes in plants | 8 | 2004 | |
| 2005/0114,920 Expression of foreign sequences in plants using transactivation system | 6 | 2004 | |
| 2006/0085,871 Systems and methods for clonal expression in plants | 4 | 2005 | |
| 2008/0241,931 System For Expression of Genes In Plants | 3 | 2008 | |
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| 5,500,360 RNA transformation vector | 48 | 1994 | |
| 5,602,242 Hybrid RNA virus | 40 | 1995 | |
| 5,846,795 RNA transformation vector | 15 | 1995 | |
| 5,627,060 Hybrid RNA virus | 27 | 1995 | |
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| 5,530,101 Humanized immunoglobulins | 602 | 1990 | |
| 5,585,089 Humanized immunoglobulins | 611 | 1995 | |
| 5,693,761 Polynucleotides encoding improved humanized immunoglobulins | 263 | 1995 | |
| 5,693,762 Humanized immunoglobulins | 473 | 1995 | |
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| 5,658,727 Heterodimeric receptor libraries using phagemids | 125 | 1994 | |
| 5,667,988 Methods for producing antibody libraries using universal or randomized immunoglobulin light chains | 42 | 1994 | |
| 5,759,817 Heterodimeric receptor libraries using phagemids | 29 | 1994 | |
| 6,852,319 Method of use of transgenic plant expressed antibodies | 7 | 2000 | |
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| 6,042,832 Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins | 18 | 1996 | |
| 6,051,239 Compositions and methods for systemic delivery of oral vaccines and therapeutic agents | 14 | 1997 | |
| 6,297,357 prb2/p130 peptide inhibitors of cdk2 kinase activity | 5 | 1998 | |
| 6,448,070 Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid | 5 | 1999 | |
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| 4,816,567 Recombinant immunoglobin preparations | 1317 | 1983 | |
| 6,331,415 Methods of producing immunoglobulins, vectors and transformed host cells for use therein | 157 | 1988 | |
| 2003/0211,568 Secreted and transmembrane polypeptides and nucleic acids encoding the same | 4 | 2001 | |
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| 5,728,300 Phytorecovery of metals using seedlings | 10 | 1996 | |
| 5,917,117 Inducing hyperaccumulation of metals in plant shoots | 16 | 1996 | |
| 5,853,576 Phytorecovery of metals using seedlings | 13 | 1997 | |
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| 6,635,805 Methods and DNA constructs for gene silencing in transgenic plants | 27 | 1999 | |
| 6,369,296 Recombinant plant viral vectors | 25 | 2000 | |
| 6,531,647 Gene silencing methods | 38 | 2000 | |
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| 5,888,789 Process for protein production in plants | 22 | 1995 | |
| 5,889,189 Process for protein production in plants | 13 | 1995 | |
| 5,994,628 Process for protein production in plants | 14 | 1995 | |
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| 5,654,184 Oral immunization by transgenic plants | 29 | 1995 | |
| 5,679,880 Oral immunization by transgenic plants | 33 | 1995 | |
| 5,686,079 Oral immunization by transgenic plants | 28 | 1995 | |
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| 5,427,908 Recombinant library screening methods | 173 | 1990 | |
| 5,580,717 Recombinant library screening methods | 181 | 1995 | |
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| 4,956,282 Mammalian peptide expression in plant cells | 134 | 1985 | |
| 5,750,871 Transformation and foreign gene expression in Brassica species | 26 | 1995 | |
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| 5,223,409 Directed evolution of novel binding proteins | 891 | 1991 | |
| 5,403,484 Viruses expressing chimeric binding proteins | 390 | 1993 | |
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| 6,077,992 Binary viral expression system in plants | 25 | 1998 | |
| 6,632,980 Binary viral expression system in plants | 10 | 1999 | |
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| 2004/0093,643 Production of pharmaceutically active proteins in sprouted seedlings | 8 | 2002 | |
| 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems | 4 | 2006 | |
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| 2005/0091,706 Recombinant viral switches for the control of gene expression in plants | 15 | 2004 | |
| 2007/0300,330 Two-Component Rna Virus-Derived Plant Expression System | 4 | 2005 | |
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| 5,922,602 Cytoplasmic inhibition of gene expression | 19 | 1994 | |
| 6,376,752 Cytoplasmic inhibition of gene expression in a plant | 5 | 1999 | |
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| 5,545,807 Production of antibodies from transgenic animals | 280 | 1994 | |
| 5,733,743 Methods for producing members of specific binding pairs | 188 | 1994 | |
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| 5,770,403 Cloning and exprssion of humanized monoclonal antibodies against human interluekin-4 | 17 | 1995 | |
| 5,705,154 Humanized monoclonal antibodies against human interleukin-4 | 36 | 1996 | |
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| 5,491,076 Expression of foreign genes using a replicating polyprotein producing virus vector | 26 | 1993 | |
| 5,766,885 Potyvirus vectors for the expression of foreign genes | 47 | 1995 | |
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| 5,939,541 Method for enhancing expression of a foreign or endogenous gene product in plants | 15 | 1997 | |
| 6,395,962 Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons | 6 | 1999 | |
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| 5,693,493 Modular assembly of antibody genes, antibodies prepared thereby and use | 31 | 1995 | |
| 5,698,417 Modular assembly of antibody genes, antibodies prepared thereby and use | 25 | 1995 | |
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| 5,698,426 Surface expression libraries of heteromeric receptors | 145 | 1995 | |
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| 2003/0135,882 Methods and means for delivering inhibitory RNA to plants and applications thereof | 15 | 2002 | |
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| 6,410,317 Recombinase-based methods for producing expression vectors and compositions for use in practicing the same | 23 | 2000 | |
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| 6,858,426 Grapevine leafroll virus (type 2) proteins and their uses | 5 | 2000 | |
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| 5,565,332 Production of chimeric antibodies - a combinatorial approach | 267 | 1994 | |
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| 6,015,692 CDC37 cell-cycle regulatory protein and uses related thereto | 5 | 1997 | |
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| 5,639,641 Resurfacing of rodent antibodies | 63 | 1992 | |
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| 2007/0178,148 Preparations of growth hormone | 4 | 2006 | |
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| 5,750,078 Process for producing humanized chimera antibody | 30 | 1995 | |
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| 5,558,864 Humanized and chimeric anti-epidermal growth factor receptor monoclonal antibodies | 70 | 1992 | |
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| 5,565,347 Transformation and foreign gene expression with plant species | 38 | 1993 | |
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| 5,702,892 Phage-display of immunoglobulin heavy chain libraries | 21 | 1995 | |
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| 2004/0088,757 Cytoplasmic gene inhibition or gene expression in transfected plants by tobraviral vector | 4 | 2003 | |
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| 6,500,644 Method for in vivo production of a mutant library in cells | 6 | 2000 | |
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| 5,175,102 Modification of plant viruses or their effects | 8 | 1989 | |
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| 5,874,087 Modified plant viruses as vectors | 12 | 1995 | |
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| 4,683,202 Process for amplifying nucleic acid sequences | 2766 | 1985 | |
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| 6,596,698 Methods of inhibiting smooth muscle cell proliferation and preventing restenosis with a vector expressing RB2/p130 | 5 | 2001 | |
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| 5,464,763 Process for the incorporation of foreign DNA into the genome of dicotyledonous plants | 69 | 1993 | |
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| 4,935,496 Mouse-human chimaeric immunoglobulin heavy chain specific for the call antigen | 26 | 1985 | |
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| 5,502,167 CDR grafted humanised chimeric T-cell antibodies | 71 | 1994 | |
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| 4,196,265 Method of producing antibodies | 243 | 1978 | |
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| 2003/0097,678 Process for converting storage reserves of dicot seeds into compositions comprising one or more gene products | 15 | 2002 | |
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| 5,447,858 Heat shock promoter and gene | 12 | 1984 | |
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| 6,399,317 Real time detection of antigens | 15 | 1999 | |
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| 6,127,145 Production of .alpha..sub.1 -antitrypsin in plants | 5 | 1998 | |
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| 2004/0092,470 Dry powder oligonucleotide formualtion, preparation and its uses | 13 | 2003 | |
| 2006/0265,787 Recombinant carrier molecule for expression, delivery and purification of target polypeptides | 8 | 2004 | |
| 2007/0292,862 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (PTGS) | 4 | 2006 | |
Patent Citation Ranking
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
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| 3.5 Year Payment | $1600.00 | $800.00 | $400.00 | Oct 6, 2013 |
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| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Oct 6, 2021 |
| Fee | Large entity fee | small entity fee | micro entity fee |
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| Surcharge - 3.5 year - Late payment within 6 months | $160.00 | $80.00 | $40.00 |
| Surcharge - 7.5 year - Late payment within 6 months | $160.00 | $80.00 | $40.00 |
| Surcharge - 11.5 year - Late payment within 6 months | $160.00 | $80.00 | $40.00 |
| Surcharge after expiration - Late payment is unavoidable | $700.00 | $350.00 | $175.00 |
| Surcharge after expiration - Late payment is unintentional | $1,640.00 | $820.00 | $410.00 |