Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
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United States of America Patent
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Apr 27, 2010
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Aug 25, 1993
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Apr 17, 1990
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Abstract
bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
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