System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
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United States of America Patent
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May 11, 2010
Grant Date -
Mar 11, 2004
app pub date -
Nov 29, 2001
filing date -
Nov 29, 2000
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Abstract
Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformity stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.
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