Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
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United States of America Patent
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May 18, 2010
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app pub date -
Jun 3, 2005
filing date -
Aug 12, 1998
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Abstract
Ligation-mediated method of recombining polynucleotides in vitro. Polynucleotides from a library are fragmented and the fragments are hybridized to an assembly template. The hybridized fragments are iteratively re-hybridized and ligated until the ends of the hybridized fragments are adjacent to the ends of other hybridized fragments on the assembly template. A final ligation produces recombined polynucleotides.
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