TM leveling methods
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United States of America Patent
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Jul 6, 2010
Grant Date -
Apr 7, 2005
app pub date -
Sep 26, 2003
filing date -
Apr 3, 1998
priority date (Note) -
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Abstract
Methods for designing an oligonucleotide sequence having a selected duplex stability are provided wherein the oligonucleotide portion comprises at least one modified base selected from universal bases, unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally having attached minor groove binders and reporter groups.
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