US Patent No: 7,754,444
Number of patents in Portfolio can not be more than 2000
Biofunctional magnetic nanoparticles for pathogen detection
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Jul 13, 2010
Issued date -
Jun 23, 2005
filing date -
11/159,882
serial no -
In Force
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Abstract
This invention provides a method of detecting pathogens comprising the steps of: (a) contacting a sufficient amount of biofunctional magnetic nanoparticles with an appropriate sample for an appropriate period of time to permit the formation of complexes between the pathogens in the sample and the nanoparticles; (b) using a magnetic field to aggregate said complexes; and (c) detecting said complexes. The method may further comprise the additional step of removing said complexes. The biofunctional magnetic nanoparticles are preferably a conjugate of vancomycin and FePt. The pathogens may be bacteria or viruses, and the sample may be a solid, liquid, or gas. Detection may involve conventional fluorescence assay, enzyme-linked immunosorbent assay (ELISA), optical microscope, electron microscope, or a combination thereof. The sensitivity of detection for the method is at least as low as 10 colony forming units (cfu) of the pathogens in one milliliter of solution within one hour.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
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| 6,254,662 Chemical synthesis of monodisperse and magnetic alloy nanocrystal containing thin films | 40 | 1999 | |
| 6,302,940 Chemical synthesis of monodisperse and magnetic alloy nanocrystal containing thin films | 20 | 2001 | |
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| 5,279,936 Method of separation employing magnetic particles and second medium | 41 | 1989 | |
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| 4,590,922 Use of ferromagnetic, paramagnetic and diamagnetic particles in the treatment of infectious diseases | 28 | 1983 | |
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| 6,262,129 Method for producing nanoparticles of transition metals | 107 | 1998 | |
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| 7,186,398 Fe/Au nanoparticles and methods | 7 | 2003 | |
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| 2006/0188,876 Binding a target substance | 5 | 2003 | |
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| 6,620,627 Increased separation efficiency via controlled aggregation of magnetic nanoparticles | 13 | 2000 | |
Patent Citation Ranking
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