Isolation of nucleic acid using colored buffers
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United States of America Patent
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Jul 13, 2010
Grant Date -
Jan 18, 2007
app pub date -
Aug 16, 2005
filing date -
Jul 16, 2005
priority date (Note) -
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Abstract
(solution becomes yellow with debris suspension); centrifuging or filtering to product a lysate filtrate; binding the lysate filtrate to a DNA binding matrix; washing; and isolating the plasmid via chromatography. The yield and quality of plasmid DNA is improved due to more consistent lysis. Errors in buffer addition are reduced by visualizing the color as buffers are added and also of changes in color of the preparation at each step.
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