Labeling reagent
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United States of America Patent
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Jul 20, 2010
Grant Date -
Sep 28, 2006
app pub date -
Mar 8, 2006
filing date -
Mar 10, 2005
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Abstract
The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.
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