Chloral-free DCA in oligonucleotide synthesis
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United States of America Patent
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Jul 20, 2010
Grant Date -
May 31, 2007
app pub date -
Jan 29, 2007
filing date -
Apr 1, 2002
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Abstract
A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is useful for making oligonucleotides that are substantially free of chloral adducts.
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