Separation systems of frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
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United States of America Patent
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Aug 10, 2010
Grant Date -
Feb 22, 2007
app pub date -
Sep 28, 2006
filing date -
Nov 29, 2000
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Abstract
Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.
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