Methods for increasing protein polyethylene glycol (PEG) conjugation
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United States of America Patent
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Sep 21, 2010
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app pub date -
Dec 10, 2007
filing date -
Mar 17, 2004
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Abstract
The present invention relates to highly conjugated proteins and methods for making such proteins. In particular, the present invention relates to methods for linking additional sites to a protein for conjugation with activated polyethylene glycol (PEG) linkers, without denaturing the protein. The invention also relates to highly conjugated proteins with decreased immunogenicity and increased circulating half-life.
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