Methods for purifying nucleic acids
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United States of America Patent
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Oct 5, 2010
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app pub date -
Jul 23, 1998
filing date -
Aug 1, 1996
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Abstract
Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.
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