Automatic threshold setting for quantitative polymerase chain reaction
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United States of America Patent
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Dec 7, 2010
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app pub date -
Dec 28, 2006
filing date -
Aug 21, 2001
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Abstract
Disclosed are systems and methods for identifying and quantitating the presence of one or more DNA species in a sample population through PCR amplification. DNA species quantitation includes a determination of a threshold fluorescence value used in the assessment of the PCR amplification reaction. Various embodiments of the present invention incorporate an enhancement function useful in selecting appropriate threshold fluorescence values and facilitate the determination of DNA concentrations by quantitative PCR based methodologies.
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