Scalable process for protein purification
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United States of America Patent
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Feb 15, 2011
Grant Date -
Feb 19, 2009
app pub date -
Jun 20, 2006
filing date -
Jun 21, 2005
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Abstract
The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations.
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